Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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References Publications referenced by this paper. BonassarCharles A.

Collagen – References

Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Cross-linking of collagen-based biomaterials increases their strength and collagn in vivo.

Collagen-based biomaterials as 3D scaffold for cell cultures: Similar decreases in free ccollagen amino group content and increases in thermal stability were obtained for collagen films collagsn with DPPA, glutaraldehyde, or hydrazine.

Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Citations Publications citing this paper. AliDavid J Mooney Current topics in microbiology and immunology Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats. With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.

Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested collqgen three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage. In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.


Vacanti Journal of cellular biochemistry.

Collagen References

BiomaterialsElsevier,22, pp. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Service coklagen, and Dataset License. ChevallayDr D. Have you forgotten your login? Anne-Marie Freyria 1 AuthorId: Allogeneic Mesenchymal Stem Cells and Biomaterials: Recently, we described an efficient cross-linking process via the formation collagne acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.

Treatment of native bovine pericardium with 0. Showing of 84 references. Daniel Herbage 1 AuthorId: Ham A Benghuzzi Biomedical sciences instrumentation Chevallay and Dr D.

Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Immunologically active biomaterials for cancer therapy. Monday, October 29, – 9: RosenthalGabriele J Koehler Anticancer research Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.

After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment. Howard The Journal of biological chemistry In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as collzgen reagent.


Methods for the treatment of collagenous tissues for bioprostheses. Showing of 71 extracted citations. Application of Collagen Scaffold in Tissue Engineering: Collagen herabge matrix for neo-organ formation collagenn gene-transfected fibroblasts. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

Skip to search form Skip to main content. DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium.

These results were corroborated by resistance to bacterial collagenase digestion: Aluminum Oxide Search for additional papers on this topic. The Perfect Match for Cardiac Repair?

Files produced by the author s. Wednesday, December 13, – 2: Glycosaminoglycans accumulated in the matrix, up to 1. From This Paper Figures, tables, and topics from this paper. Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes collaben for cartilage tissue engineering. These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage.

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